Since totally different morphology of cells of epithelial sort is observed in two forms of tissue, the share of immunoractive cells (labelling index) was calculated in the consultant photos of every sample and used for the comparison of the two types of colorectal tissue. Right here, we mixed an environment friendly method for protein extraction from tissue samples with lysine-acetylated peptide immunoprecipitation and excessive-accuracy tandem mass spectrometric (MS/MS) measurements, which allowed us to generate an atlas of lysine acetylation sites in sixteen totally different rat tissues.
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One of the building blocks of protein, lysine is supplied by many foods—notably purple meats, fish and dairy products. Spectrometer parameters had been as follows: polarity mode, positive
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